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Turella P, Cerella C, Filomeni G, Bullo A, De Maria F, Ghibelli L et al
The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)
The tyrosine derivative, 3-CT, is a stable marker of inflammation that is generated from hypochlorous acid, a potent chlorinating oxidant derived from myeloperoxidase in activated immune cells during an inflammatory response
Compared to sgRNA controls, sg CBS significantly reduced intracellular cystathionine, cysteine, and GSH levels
Cells were post-fixed for 1 h with 1% osmium tetroxide (Agar Scientific) in CACO buffer, dehydrated in a graded series of methanol and embedded in LR white resin
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