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Firstly, roGFP-iL was expressed as described above for 16 h at 20 C, cells were harvested, washed in HEPES buffer (40 mM, pH 7.4) and adjusted to an OD 600 of 1.0 in HEPES buffer

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When Zueler injected this preparation into diabetic dogs he encouragingly observed that it significantly reduced the concentration of sugar and ketone, acidic substances produced in the liver resulting from the breakdown of fats, in their urine