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Furthermore, FMO3 is regulated by farnesoid X receptor (FXR), a nuclear receptor activated by bile acid (Lefebvre et al., 2009), and injection of FXR ligands induced the expression of FMO3 and the production of TMAO in mice ( TMAO levels were significantly associated with body mass index (BMI) in healthy adults with different risk factors (Wang et al., 2016), and studies in humans and mice have shown that plasma TMAO levels increased with ageing (Wang et al., 2014

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